Composite

Part:BBa_K1256009:Design

Designed by: Pei-Hong Chen   Group: iGEM14_Mingdao   (2014-06-09)

pSB1C3-Plac-SS-RNase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2117
    Illegal PstI site found at 2438
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2117
    Illegal NheI site found at 290
    Illegal PstI site found at 2438
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2117
    Illegal BamHI site found at 2718
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2117
    Illegal PstI site found at 2438
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2117
    Illegal PstI site found at 2438
    Illegal NgoMIV site found at 792
    Illegal NgoMIV site found at 1381
    Illegal NgoMIV site found at 1961
    Illegal NgoMIV site found at 2059
    Illegal NgoMIV site found at 2830
    Illegal AgeI site found at 1169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1295
    Illegal SapI.rc site found at 2744


Design Notes

Source

References